The effect of enzyme and substrate concentration on a reaction rate

Factors affecting Enzyme Activity Factors affecting Enzyme Activity The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme.

The effect of enzyme and substrate concentration on a reaction rate

Science What Effects of Concentrations of… An enzyme is described as a biological catalyst that speeds up the rate of a chemical reaction.

In order for an enzyme to perform its given job, it needs what is known as a substrate to bind to the active site of the enzyme so that the enzyme can speed up the reaction of the substrate. In this given experiment, it was to be tested what impact the concentration level of substrate will have on the reaction rate.

The enzyme catalase, found in potato juice, was used for the catalyst along with a substrate known as hydrogen peroxide H2O2.

The effect of enzyme and substrate concentration on a reaction rate

The job of catalase in this experiment was to accelerate the breakdown of hydrogen peroxide into water and oxygen gas. The given chart and graph shows the different concentration levels of substrate that were used, along with the time it took for the reaction to occur.

Enzyme Concentration

An enzyme is supposed to speed up the reaction, but our observations shows that the concentration of the substrate also had an effect on how fast the reaction could occur. When there was zero concentrate of hydrogen peroxide, meaning the solvent was only water, there was no reaction with the catalase.

As the concentration of hydrogen peroxide increases, the rate of the reaction began to increase. This occurred because adding amounts of hydrogen peroxide gave the catalase the opportunity to break down the hydrogen peroxide, where as when there was no H2O2 present, there was nothing for the catalase to react with.

The catalase needs its specific substrate to bind with in order for a reaction to occur. The shape of the chart itself shows the decrease in needed reaction time, proving our analysis to be correct.

If we had continued to test out different concentrations of our substrate, the reaction time would have continued to decrease until the enzyme hit what is known as the saturation level, which means all the enzymes are working and the reaction cannot continue to increase because there is no location at that time for the substrates to bind to.

For this experiment, the saturation point was not reached, but could have if the experiment had continued. From our observations, it could be conclude that the higher the concentration of substrate, Hydrogen Peroxide there was in the reaction, the less time was needed for the reaction to occur.This means, enzyme concentration becomes a limiting factor, which means any increase in substrate concentration does not increase the rate of reaction.

Another substance that can affect the activity of enzyme is inhibitor such as sarin/5(6). Concentration Effects Summary. Enzyme reaction rates have set limits: they can't drop past zero (no reaction) and they can't speed up past %.

When there is a ratio of enzyme and substrate, the reaction is going as fast as it can. Add more enzyme, and there won't be substrate for it to react with. Objective: To analyze the effect of substrate concentration on the activity of enzymes. Theory: Enzymes are protein molecules that act as biological catalysts by increasing the rate of reactions without changing the overall process.

An enzyme is described as a biological catalyst that speeds up the rate of a chemical reaction. In order for an enzyme to perform its given job, it needs what is known as a substrate to bind to the active site of the enzyme so that the enzyme can speed up the reaction of the substrate.

Determination of the effect of enzyme concentration on catalysis using starch an amylase. INTRODUCTION. Enzymes are said to be catalytic proteins which increases the rate of a chemical reaction without being altered in the process of that reaction. [1] A substrate is a substance which an enzyme acts upon.

The Effect of Substrate Concentration on the Activity of the Enzyme Catalase Words | 8 Pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.

the effect of changing conditions in enzyme catalysis